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Journal: Research
Article Title: NIR-Activated ICG-Loaded M2 Macrophage Exosomes Ameliorate Periodontitis via Targeting Infection Inflammation and Oxidative Stress
doi: 10.34133/research.1207
Figure Lengend Snippet: Indocyanine green-loaded M2 macrophage-derived exosomes (ICG@M2-exos) plus near-infrared (NIR) irradiation exhibits a time-dependent dual role in reactive oxygen species (ROS) regulation. (A) A dose- and time-dependent increase in intracellular ROS levels was detected upon H 2 O 2 treatment (0.1 to 1.0 mM, 1 to 6 h). The 0.5 mM, 4-h condition (indicated) was chosen for subsequent studies. (B) Corresponding cell viability assessment for the selected condition, verifying a suitable window for intervention studies. (C) Fluorescence images showing intracellular ROS levels (green) under different conditions. (D) Quantitative fluorescence intensity analysis. Values are means ± SD ( n = 3). *** P < 0.001. a.u., arbitrary units.
Article Snippet: Intracellular ROS detection:
Techniques: Derivative Assay, Irradiation, Fluorescence
Journal: MedComm
Article Title: Ipriflavone From Aquilaria malaccensis Lam. Exosome‐Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia‐Inducible Factor‐α (HIF‐α) Hydroxylation Thereby Alleviating Hypoxia‐Induced Gastrointestinal Mucosal Ferroptosis
doi: 10.1002/mco2.70722
Figure Lengend Snippet: Oral administration of AELNs ameliorated gastric and small intestinal mucosal ferroptosis caused by hypoxia in mice. The mice were housed in a vented hypoxic chamber for 3 days ( n = 10 mice in each group). They received PBS or AELNs (1 × 10 5 particles) via oral gavage on Day 1 and were euthanized on Day 3. Representative photographs showing the microstructures of the murine gastric (A) and small intestinal mucosal cells (B) under transmission electron microscopy (TEM). The red arrows indicate shrunken mitochondria (scale bar: overview, 1 µm; inset, 500 nm). Quantification of Fe 2+ , reactive oxygen species (ROS), lipid peroxidation (LPO), and 4‐hydroxynonenal (4‐HNE) of the gastric (C) and small intestinal (D) mucosa ( n = 10). Western blot analysis of the indicated protein levels in the gastric (E) and small intestinal (F) mucosa assessed by Western blot. (G) Cytokines in the gastric (upper) and small intestinal (lower) mucosa ( n = 10). Error bars indicate mean ± SEM. One‐way ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; ns, not significant.
Article Snippet: ROS, 4‐HNE, and
Techniques: Transmission Assay, Electron Microscopy, Western Blot